Practical spectroscopy

Practical Course Biophysics: Fluorescence Correlation Spectroscopy

Near Infrared Spectroscopy: Fundamentals, Practical Aspects and Analytical Applications

Este trabalho visa prover uma revisão da teoria da Espectroscopia no Infravermelho Próximo (NIR) e das suas aplicações no campo das Ciências Analíticas. Ele é dirigido ao leitor que não possui um conhecimento profundo sobre espectroscopia vibracional mas que gostaria de ser introduzido às potencialidades analíticas desta técnica fascinante e, ao mesmo tempo, se tornar consciente das suas limita...

Theory and practical recommendations for autocorrelation-based image correlation spectroscopy.

Image correlation spectroscopy (ICS) is a powerful technique for detecting arrangement of fluorophores in images. This tutorial gives background into the mathematical underpinnings of ICS, specifically image autocorrelation. The effects of various artifacts and image processing steps, including background subtraction, noise, and image morphology were examined analytically and their effects on I...

Ibn Sīnā’s Practical Philosophy

This article is a brief review of Ibn Sīnā’s practical philosophy. It begins with a discussion of the Platonic, Aristotelian, and Neo-Platonic influences on Ibn Sīnā’s practical philosophy, as well as the influence of Fārābī and of Islamic religious teachings. The creative synthesis invented by Ibn Sīnā requires a particular view of the relation between religion and philosophy in such a manner ...

Single-molecule force-clamp spectroscopy: dwell time analysis and practical considerations.

Single-molecule force-clamp spectroscopy has become a powerful tool for studying protein folding/unfolding, bond rupture, and enzymatic reactions. Different methods have been developed to analyze force-clamp spectroscopy data on polyproteins to obtain kinetic parameters characterizing the mechanical unfolding of proteins, which are often modeled as a two-state process (a Poisson process). Howev...